Effect of 24 weeks of intensification with a CCR5-antagonist on the decay of the HIV-1 latent reservoir
نویسندگان
چکیده
Methods The intensification study with an entry inhibitor (maraviroc-MVC) included patients with stable antiretroviral therapy (ART) with three or more drugs, viral load (VL) of <50 copies/ml for two or more years, CD4 count of >350 cells/mm and CCR5-tropism. Latently infected resting CD4 cells were determined at baseline, week 12 and week 24, with further analyses planned at week 36 and week 48. Highly purified resting CD4 cells were isolated using magnetic beads rendering ~20 million and plated in replicate dilutions and stimulated in a limiting dilution culture assay, with irradiated PBMCs from a seronegative donor. Cells containing HIV-replicationcompetent were quantified by HIV-1 p24 Antigen Assay (results in infective units per million).
منابع مشابه
Intensification of Antiretroviral Therapy with a CCR5 Antagonist in Patients with Chronic HIV-1 Infection: Effect on T Cells Latently Infected
OBJECTIVE The primary objective was to assess the effect of MVC intensification on latently infected CD4(+) T cells in chronically HIV-1-infected patients receiving antiretroviral therapy. METHODS We performed an open-label pilot phase II clinical trial involving chronically HIV-1-infected patients receiving stable antiretroviral therapy whose regimen was intensified with 48 weeks of maraviro...
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عنوان ژورنال:
دوره 13 شماره
صفحات -
تاریخ انتشار 2010